Pause locally, splice globally.
نویسندگان
چکیده
Most eukaryotic protein-coding transcripts contain introns, which vary in number and position along the transcript body. Intron removal through pre-mRNA splicing is tightly linked to transcription by RNA polymerase II as it translocates along each gene. Here, we review recent evidence that transcription and splicing are functionally coupled. We focus on how RNA polymerase II elongation rates impact splicing through local regulation and transcriptional pausing within genes. Emerging concepts of how splicing-related changes in elongation might be achieved are highlighted. We place the interplay between transcription and splicing in the context of chromatin where nucleosome positioning influences elongation, and histone modifications participate directly in the recruitment of splicing regulators to nascent transcripts.
منابع مشابه
Built-In Learner Participation Potential of Locally- and Globally-Designed ELT Materials
This study aims at empirically measuring a universal criterion for materials evaluation, i.e., learning opportunities, in a locally- and a globally-designed materials. Adopting the conceptual framework of sociocultural theory and its conceptualization of learning as participation (Donato, 2000), the researchers utilized the methodological power of conversation analysis to examine how opportunit...
متن کاملClock Synchronization through Handshake Signalling
We present a method for synchronizing pausible clocks in GALS (Globally Asynchronous, Locally Synchronous) systems. In contrast to most conventional GALS schemes the method is not based on including in each ring oscillator a synchronizing element (such as for instance an arbiter) which on one side can pause the clock and on the other side offers a handshake interface. Instead, we propose a sche...
متن کاملNucleosomal fluctuations govern the transcription dynamics of RNA polymerase II.
RNA polymerase II (Pol II) must overcome the barriers imposed by nucleosomes during transcription elongation. We have developed an optical tweezers assay to follow individual Pol II complexes as they transcribe nucleosomal DNA. Our results indicate that the nucleosome behaves as a fluctuating barrier that locally increases pause density, slows pause recovery, and reduces the apparent pause-free...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Trends in cell biology
دوره 21 6 شماره
صفحات -
تاریخ انتشار 2011